Host: |
Rabbit |
Applications: |
WB/IF |
Reactivity: |
Human/Mouse/Rat |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Rabbit polyclonal antibody anti-NPHP4 (1247-1426) is suitable for use in Western Blot and Immunofluorescence research applications. |
Clonality: |
Polyclonal |
Conjugation: |
Unconjugated |
Isotype: |
IgG |
Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
Purification: |
Affinity purification |
Dilution Range: |
WB 1:1000-1:3000IF 1:50-1:200 |
Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: |
NPHP4 |
Gene ID: |
261734 |
Uniprot ID: |
NPHP4_HUMAN |
Immunogen Region: |
1247-1426 |
Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1247-1426 of human NPHP4 (NP_055917.1). |
Immunogen Sequence: |
RLSLVLRGTQTVRKVRAFTS HPQELKTDPKGVFVLPPRGV QDLHVGVRPLRAGSRFVHLN LVDVDCHQLVASWLVCLCCR QPLISKAFEIMLAAGEGKGV NKRITYTNPYPSRRTFHLHS DHPELLRFREDSFQVGGGET YTIGLQFAPSQRVGEEEILI YINDHEDKNEEAFCVKVIYQ |
Tissue Specificity | Expressed in kidney, skeletal muscle, heart and liver, and to a lesser extent in brain and lung. |
Function | Involved in the organization of apical junctions.the function is proposed to implicate a NPHP1-4-8 module. Does not seem to be strictly required for ciliogenesis. Required for building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells. Seems to recruit INT to basal bodies of motile cilia which subsequently interacts with actin-modifying proteins such as DAAM1. In cooperation with INVS may down-regulate the canonical Wnt pathway and promote the Wnt-PCP pathway by regulating expression and subcellular location of disheveled proteins. Stabilizes protein levels of JADE1 and promotes its translocation to the nucleus leading to cooperative inhibition of canonical Wnt signaling. Acts as negative regulator of the hippo pathway by association with LATS1 and modifying LATS1-dependent phosphorylation and localization of WWTR1/TAZ. |
Protein Name | Nephrocystin-4Nephroretinin |
Database Links | Reactome: R-HSA-2028269Reactome: R-HSA-5620912 |
Cellular Localisation | CytoplasmCytoskeletonCilium Basal BodyMicrotubule Organizing CenterCentrosomeCell JunctionTight JunctionNucleusIn Cultured Renal CellsIt Localizes Diffusely In The Cytoplasm ButAs Cells Approach ConfluenceIt Accumulates To Basolateral Tight JunctionsLocalizes To The Ciliary Transition ZoneIn The Retinal Photoreceptor Cell LayerLocalizes At The Connecting Cilium |
Alternative Antibody Names | Anti-Nephrocystin-4 antibodyAnti-Nephroretinin antibodyAnti-NPHP4 antibodyAnti-KIAA0673 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance