Anti-NDC80 antibody (351-400 aa) (STJ93486)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human KNTC2 at the amino acid range 351-400
General Information
| Short Description | Rabbit polyclonal anti-Kinetochore protein NDC80 homolog (351-400 aa) for use in WB, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NDC80 |
| Gene ID | 10403 |
| Uniprot ID | NDC80_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human KNTC2 at the amino acid range 351-400 |
| Immunogen Region | 351-400 aa |
| Specificity | Hec1 Polyclonal Antibody detects endogenous levels of Hec1 protein. |
Additional Info
| Function | Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules. |
| Protein Name | Kinetochore Protein Ndc80 HomologHighly Expressed In Cancer ProteinKinetochore Protein Hec1Hshec1Kinetochore-Associated Protein 2Retinoblastoma-Associated Protein Hec |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreLocalizes To Kinetochores From Late Prophase To AnaphaseLocalizes Specifically To The Outer Plate Of The Kinetochore |
| Alternative Antibody Names | Anti-Kinetochore Protein Ndc80 Homolog antibodyAnti-Highly Expressed In Cancer Protein antibodyAnti-Kinetochore Protein Hec1 antibodyAnti-Hshec1 antibodyAnti-Kinetochore-Associated Protein 2 antibodyAnti-Retinoblastoma-Associated Protein Hec antibodyAnti-NDC80 antibodyAnti-HEC antibodyAnti-HEC1 antibodyAnti-KNTC2 antibody |
Information sourced from Uniprot.org