• Western blot analysis of lysates from Jurkat cells, using KNTC2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of Jurkat cells using Hec1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunofluorescence analysis of HUVEC cells, using KNTC2 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of Hela cell. 1, Hec1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Kif 7 monoclonal antibody (3F8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-NDC80 antibody (351-400 aa) (STJ93486)

SKU:
STJ93486

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Kinetochore protein NDC80 homolog (351-400 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDC80
Gene ID: 10403
Uniprot ID: NDC80_HUMAN
Immunogen Region: 351-400 aa
Specificity: Hec1 Polyclonal Antibody detects endogenous levels of Hec1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human KNTC2 at the amino acid range 351-400
Function Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.
Protein Name Kinetochore Protein Ndc80 Homolog
Highly Expressed In Cancer Protein
Kinetochore Protein Hec1
Hshec1
Kinetochore-Associated Protein 2
Retinoblastoma-Associated Protein Hec
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Localizes To Kinetochores From Late Prophase To Anaphase
Localizes Specifically To The Outer Plate Of The Kinetochore
Alternative Antibody Names Anti-Kinetochore Protein Ndc80 Homolog antibody
Anti-Highly Expressed In Cancer Protein antibody
Anti-Kinetochore Protein Hec1 antibody
Anti-Hshec1 antibody
Anti-Kinetochore-Associated Protein 2 antibody
Anti-Retinoblastoma-Associated Protein Hec antibody
Anti-NDC80 antibody
Anti-HEC antibody
Anti-HEC1 antibody
Anti-KNTC2 antibody

Information sourced from Uniprot.org

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