| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MTF2 (1-190) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MTF2 |
| Gene ID: |
22823 |
| Uniprot ID: |
MTF2_HUMAN |
| Immunogen Region: |
1-190 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MTF2 (NP_001157864.1). |
| Immunogen Sequence: |
MRDSTGAGNSLVHKRSPLRR NQKTPTSLTKLSLQDGHKAK KPACKFEEGQDVLARWSDGL FYLGTIKKINILKQSCFIIF EDSSKSWVLWKDIQTGATGS GEMVCTICQEEYSEAPNEMV ICDKCGQGYHQLCHTPHIDS SVIDSDEKWLCRQCVFATTT KRGGALKKGPNAKALQVMKQ TLPYSVADLE |
| Function | Polycomb group (PcG) protein that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex, thus enhancing PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element (MRE) of MT1A gene promoter. |
| Protein Name | Metal-Response Element-Binding Transcription Factor 2Metal Regulatory Transcription Factor 2Metal-Response Element Dna-Binding Protein M96Polycomb-Like Protein 2Hpcl2 |
| Database Links | Reactome: R-HSA-212300 |
| Cellular Localisation | NucleusLocalizes To Chromatin As Part Of The Prc2 Complex |
| Alternative Antibody Names | Anti-Metal-Response Element-Binding Transcription Factor 2 antibodyAnti-Metal Regulatory Transcription Factor 2 antibodyAnti-Metal-Response Element Dna-Binding Protein M96 antibodyAnti-Polycomb-Like Protein 2 antibodyAnti-Hpcl2 antibodyAnti-MTF2 antibodyAnti-PCL2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and ATF2 knockout (KO) 293T cells, using [KO Validated] ATF2 Rabbit polyclonal antibody (STJ22702) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/99177/383848/STJ22702_1__38112.1713152438.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ATF2 knockout (KO) 293T cells, using [KO Validated] ATF2 Rabbit polyclonal antibody (STJ22702) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")