![Immunofluorescence analysis ofย MCF7 cells usingย [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) atย aย dilution ofย 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178259/370575/STJ11105615_1__57963.1713136524.jpg?c=1 "Immunofluorescence analysis ofย MCF7 cells usingย [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) atย aย dilution ofย 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from MCF7 cells usingย [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) atย 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ย Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178259/370576/STJ11105615_2__81408.1713136525.jpg?c=1 "Western blot analysis of lysates from MCF7 cells usingย [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) atย 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ย Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from wild type (WT) and MRPL9 knockdown (KD) HeLa cellsย usingย [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) atย 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ย Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time:ย 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178259/370577/STJ11105615_3__64653.1713136526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MRPL9 knockdown (KD) HeLa cellsย usingย [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) atย 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ย Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time:ย 10s.")
![Immunofluorescence analysis ofย MCF7 cells usingย [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) atย aย dilution ofย 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/178259/370575/STJ11105615_1__57963.1713136524.jpg?c=1 "Immunofluorescence analysis ofย MCF7 cells usingย [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) atย aย dilution ofย 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-MRPL9 antibody (53-267) (STJ11105615)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Large Ribosomal Subunit Protein Bl9m (53-267) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MRPL9 |
| Gene ID | 65005 |
| Uniprot ID | RM09_HUMAN |
| Immunogen Region | 53-267 |
| Immunogen Sequence | TVIVERWWKVPLAGEGRKPR LHRRHRVYKLVEDTKHRPKE NLELILTQSVENVGVRGDLV SVKKSLGRNRLLPQGLAVYA SPENKKLFEEEKLLRQEGKL EKIQTKAGEATVKFLKSCRL EVGMKNNVKWELNPEIVARH FFKNLGVVVAPHTLKLPEEP ITRWGEYWCEVTVNGLDTVR VPMSVVNFEKPKTKRYKYWL AQQAAKAMAPTSPQI |
| Specificity | Recombinant Protein corresponding to a sequence within amino acids 53-267 of human MRPL9 (NP_113608.1). |
Additional Info
| Protein Name | Large Ribosomal Subunit Protein Bl9m39s Ribosomal Protein L9 - MitochondrialL9mtMrp-L9 |
| Database Links | Reactome: R-HSA-5368286Reactome: R-HSA-5389840Reactome: R-HSA-5419276 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein Bl9m antibodyAnti-39s Ribosomal Protein L9 - Mitochondrial antibodyAnti-L9mt antibodyAnti-Mrp-L9 antibodyAnti-MRPL9 antibody |
Information sourced from Uniprot.org