![Immunofluorescence analysis of MCF7 cells using [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178259/370575/STJ11105615_1__57963.1713136524.jpg?c=1 "Immunofluorescence analysis of MCF7 cells using [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from MCF7 cells using [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178259/370576/STJ11105615_2__81408.1713136525.jpg?c=1 "Western blot analysis of lysates from MCF7 cells using [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from wild type (WT) and MRPL9 knockdown (KD) HeLa cells using [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178259/370577/STJ11105615_3__64653.1713136526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MRPL9 knockdown (KD) HeLa cells using [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunofluorescence analysis of MCF7 cells using [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/178259/370575/STJ11105615_1__57963.1713136524.jpg?c=1 "Immunofluorescence analysis of MCF7 cells using [KD Validated] MRPL9 Rabbit polyclonal antibody (STJ11105615) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MRPL9 |
| Gene ID: | 65005 |
| Uniprot ID: | RM09_HUMAN |
| Immunogen Region: | 53-267 |
| Specificity: | Recombinant Protein corresponding to a sequence within amino acids 53-267 of human MRPL9 (NP_113608.1). |
| Immunogen Sequence: | TVIVERWWKVPLAGEGRKPR LHRRHRVYKLVEDTKHRPKE NLELILTQSVENVGVRGDLV SVKKSLGRNRLLPQGLAVYA SPENKKLFEEEKLLRQEGKL EKIQTKAGEATVKFLKSCRL EVGMKNNVKWELNPEIVARH FFKNLGVVVAPHTLKLPEEP ITRWGEYWCEVTVNGLDTVR VPMSVVNFEKPKTKRYKYWL AQQAAKAMAPTSPQI |
| Protein Name | Large Ribosomal Subunit Protein Bl9m39s Ribosomal Protein L9 - MitochondrialL9mtMrp-L9 |
| Database Links | Reactome: R-HSA-5368286Reactome: R-HSA-5389840Reactome: R-HSA-5419276 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein Bl9m antibodyAnti-39s Ribosomal Protein L9 - Mitochondrial antibodyAnti-L9mt antibodyAnti-Mrp-L9 antibodyAnti-MRPL9 antibody |
Information sourced from Uniprot.org