• Immunofluorescence analysis of NIH/3T3 cells using MGRN1 Rabbit polyclonal antibody (STJ11103981) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Rat heart, using MGRN1 antibody (STJ11103981) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MGRN1 antibody (1-538) (STJ11103981)

SKU:
STJ11103981

$151.88 - $705.38
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E3 Ubiquitin-Protein Ligase Mgrn1 (1-538) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MGRN1
Gene ID: 23295
Uniprot ID: MGRN1_HUMAN
Immunogen Region: 1-538
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-538 of human MGRN1 (O60291).
Immunogen Sequence: MGSILSRRIAGVEDIDIQAN SAYRYPPKSGNYFASHFFMG GEKFDTPHPEGYLFGENMDL NFLGSRPVQFPYVTPAPHEP VKTLRSLVNIRKDSLRLVRY KDDADSPTEDGDKPRVLYSL EFTFDADARVAITIYCQASE EFLNGRAVYSPKSPSLQSET VHYKRGVSQQFSLPSFKIDF SEWKDDELNFDLDRGVFPVV IQAVVDEGDVVEVTGHAHVL LAAFEKHMDGSFSVKPLKQ
Post Translational Modifications Autoubiquitinated in vitro.
Function E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Acts also as a negative regulator of hedgehog signaling.
Protein Name E3 Ubiquitin-Protein Ligase Mgrn1
Mahogunin Ring Finger Protein 1
Ring Finger Protein 156
Ring-Type E3 Ubiquitin Transferase Mgrn1
Database Links Reactome: R-HSA-983168
Cellular Localisation Early Endosome
The Endosomal Localization Is Dependent On The Interaction With Tsg101
Isoform 1: Cytoplasm
Cytosol
Nucleus
Translocation From The Cytosol To The Nucleus Is Seen Only In The Presence Of Mc1r And Mc4r
But Not Tbxa2r
Excluded From Nucleoli
Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Cell Membrane
Isoform 4: Cytoplasm
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Mgrn1 antibody
Anti-Mahogunin Ring Finger Protein 1 antibody
Anti-Ring Finger Protein 156 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Mgrn1 antibody
Anti-MGRN1 antibody
Anti-KIAA0544 antibody
Anti-RNF156 antibody

Information sourced from Uniprot.org

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