• Western blot analysis of various lysates, using MGEA5 antibody (STJ111534) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using MGEA5 rabbit polyclonal antibody (STJ111534) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using MGEA5 rabbit polyclonal antibody (STJ111534) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-MGEA5 antibody (380-580) (STJ111534)

SKU:
STJ111534

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MGEA5 (380-580) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OGA
Gene ID: 10724
Uniprot ID: OGA_HUMAN
Immunogen Region: 380-580
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-580 of human MGEA5 (NP_001135906.1).
Immunogen Sequence: YQEPIMSQGAALSGEPTTLT KEEEKKQPDEEPMDMVVEKQ EETDHKNDNQILSEIVEAKM AEELKPMDTDKESIAESKSP EMSMQEDCISDIAPMQTDEQ TNKEQFVPGPNEKPLYTAEP VTLEDLQLLADLFYLPYEHG PKGAQMLREFQWLRANSSVV SVNCKGKDSEKIEEWRSRAA KFEEMCGLVMGMFTRLSNCA N
Post Translational Modifications Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other.cleavage does not decrease enzyme activity.
Function Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
Protein Name Protein O-Glcnacase
Oga
Beta-N-Acetylglucosaminidase
Beta-N-Acetylhexosaminidase
Beta-Hexosaminidase
Meningioma-Expressed Antigen 5
N-Acetyl-Beta-D-Glucosaminidase
N-Acetyl-Beta-Glucosaminidase
Nuclear Cytoplasmic O-Glcnacase And Acetyltransferase
Ncoat
Cellular Localisation Isoform 3: Nucleus
Isoform 1: Cytoplasm
Alternative Antibody Names Anti-Protein O-Glcnacase antibody
Anti-Oga antibody
Anti-Beta-N-Acetylglucosaminidase antibody
Anti-Beta-N-Acetylhexosaminidase antibody
Anti-Beta-Hexosaminidase antibody
Anti-Meningioma-Expressed Antigen 5 antibody
Anti-N-Acetyl-Beta-D-Glucosaminidase antibody
Anti-N-Acetyl-Beta-Glucosaminidase antibody
Anti-Nuclear Cytoplasmic O-Glcnacase And Acetyltransferase antibody
Anti-Ncoat antibody
Anti-OGA antibody
Anti-HEXC antibody
Anti-KIAA0679 antibody
Anti-MEA5 antibody
Anti-MGEA5 antibody

Information sourced from Uniprot.org

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