• Immunohistochemistry analysis of paraffin-embedded mouse spleen using MEF2A+MEF2C Rabbit monoclonal antibody (STJ11103504) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using MEF2A+MEF2C Rabbit monoclonal antibody (STJ11103504) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using MEF2A+MEF2C antibody (STJ11103504) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MEF2A+MEF2C antibody (250-473) [S4MR] (STJ11103504)

SKU:
STJ11103504

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MEF2A+MEF2C (250-473) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 250-473
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-473 of human MEF2A+MEF2C (Q06413).
Immunogen Sequence: NRKPDLRVLIPPGSKNTMPS VSEDVDLLLNQRINNSQSAQ SLATPVVSVATPTLPGQGMG GYPSAISTTYGTEYSLSSAD LSSLSGFNTASALHLGSVTG WQQQHLHNMPPSALSQLGAC TSTHLSQSSNLSLPSTQSLN IKSEPVSPPRDRTTTPSRYP QHTRHEAGRSPVDSLSSCSS SYDGSDREDHRNEFHSPIGL TRPSPDERESPSVKRMRLSE GWAT
Background The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development.

Information sourced from Uniprot.org

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