Anti-MCAM antibody (26-646) (STJ115862)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MCAM (26-646) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MCAM |
| Gene ID | 4162 |
| Uniprot ID | MUC18_HUMAN |
| Immunogen Region | 26-646 |
| Immunogen Sequence | GEAEQPAPELVEVEVGSTAL LKCGLSQSQGNLSHVDWFSV HKEKRTLIFRVRQGQGQSEP GEYEQRLSLQDRGATLALTQ VTPQDERIFLCQGKRPRSQE YRIQLRVYKAPEEPNIQVNP LGIPVNSKEPEEVATCVGRN GYPIPQVIWYKNGRPLKEEK NRVHIQSSQTVESSGLYTLQ SILKAQLVKEDKDAQFYCEL NYRLPSGNHMKESREVTVPV FYPTEKVWLEVEPVGMLKE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 26-350 of human CD146/MCAM (NP_006491.2). |
Additional Info
| Tissue Specificity | Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis. |
| Function | Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. |
| Protein Name | Cell Surface Glycoprotein Muc18Cell Surface Glycoprotein P1h12Melanoma Cell Adhesion MoleculeMelanoma-Associated Antigen A32Melanoma-Associated Antigen Muc18S-Endo 1 Endothelial-Associated AntigenCd Antigen Cd146 |
| Database Links | Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013408Reactome: R-HSA-9013423Reactome: R-HSA-9035034 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Cell Surface Glycoprotein Muc18 antibodyAnti-Cell Surface Glycoprotein P1h12 antibodyAnti-Melanoma Cell Adhesion Molecule antibodyAnti-Melanoma-Associated Antigen A32 antibodyAnti-Melanoma-Associated Antigen Muc18 antibodyAnti-S-Endo 1 Endothelial-Associated Antigen antibodyAnti-Cd Antigen Cd146 antibodyAnti-MCAM antibodyAnti-MUC18 antibody |
Information sourced from Uniprot.org