| Short Description : | Rabbit polyclonal KLHDC3 (1-382) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:1000-1:5000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | KLHDC3 |
| Gene ID: | 116138 |
| Uniprot ID: | KLDC3_HUMAN |
| Immunogen Region: | 1-382 |
| Immunogen Sequence: | MLRWTVHLEGGPRRVNHAAV AVGHRVYSFGGYCSGEDYET LRQIDVHIFNAVSLRWTKLP PVKSAIRGQAPVVPYMRYGH STVLIDDTVLLWGGRNDTEG ACNVLYAFDVNTHKWFTPRV SGTVPGARDGHSACVLGKIM YIFGGYEQQADCFSNDIHKL DTSTMTWTLICTKGSPARWR DFHSATMLGSHMYVFGGRAD RFGPFHSNNEIYCNRIRVFD TRTEAWLDCPPTPVLPEGR |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-382 of human KLHDC3 (NP_476502.1). |
| Function | Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The CRL2(KLHDC3) complex specifically recognizes proteins with a glycine (Gly) at the C-terminus, leading to their ubiquitination and degradation: recognizes the C-terminal -Arg-(Xaa)n-Arg-Gly, -Arg-(Xaa)n-Lys-Gly, and -Arg-(Xaa)n-Gln-Gly degrons. The CRL2(KLHDC3) complex mediates ubiquitination and degradation of truncated SELENOV and SEPHS2 selenoproteins produced by failed UGA/Sec decoding, which end with a glycine. May be involved in meiotic recombination process. |
| Protein Name | Kelch Domain-Containing Protein 3Testis Intracellular Mediator Protein |
| Database Links | Reactome: R-HSA-381038 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Kelch Domain-Containing Protein 3 antibodyAnti-Testis Intracellular Mediator Protein antibodyAnti-KLHDC3 antibodyAnti-PEAS antibody |
Information sourced from Uniprot.org
