• Western blot analysis of various lysates using KIRREL Rabbit polyclonal antibody (STJ116495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-KIRREL antibody (17-200) (STJ116495)

SKU:
STJ116495

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KIRREL (17-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIRREL1
Gene ID: 55243
Uniprot ID: KIRR1_HUMAN
Immunogen Region: 17-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 17-200 of human KIRREL (NP_060710.3).
Immunogen Sequence: QGTQTRFSQEPADQTVVAGQ RAVLPCVLLNYSGIVQWTKD GLALGMGQGLKAWPRYRVVG SADAGQYNLEITDAELSDDA SYECQATEAALRSRRAKLTV LIPPEDTRIDGGPVILLQAG TPHNLTCRAFNAKPAATIIW FRDGTQQEGAVASTELLKDG KRETTVSQLLINPTDLDIGR VFTC
Tissue Specificity Abundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli.
Post Translational Modifications Phosphorylation probably regulates the interaction with NSH2. Phosphorylated at Tyr-605 and Tyr-606 by FYN, leading to GRB2 binding. N-glycosylated.
Function Required for proper function of the glomerular filtration barrier. It is involved in the maintenance of a stable podocyte architecture with interdigitating foot processes connected by specialized cell-cell junctions, known as the slit diaphragm. It is a signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1.
Protein Name Kin Of Irre-Like Protein 1
Kin Of Irregular Chiasm-Like Protein 1
Nephrin-Like Protein 1
Database Links Reactome: R-HSA-373753
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Predominantly Located At Podocyte Slit Diaphragm
Alternative Antibody Names Anti-Kin Of Irre-Like Protein 1 antibody
Anti-Kin Of Irregular Chiasm-Like Protein 1 antibody
Anti-Nephrin-Like Protein 1 antibody
Anti-KIRREL1 antibody
Anti-KIRREL antibody
Anti-NEPH1 antibody

Information sourced from Uniprot.org

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