Anti-KIRREL antibody (17-200) (STJ116495)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal KIRREL (17-200) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KIRREL1 |
| Gene ID | 55243 |
| Uniprot ID | KIRR1_HUMAN |
| Immunogen Region | 17-200 |
| Immunogen Sequence | QGTQTRFSQEPADQTVVAGQ RAVLPCVLLNYSGIVQWTKD GLALGMGQGLKAWPRYRVVG SADAGQYNLEITDAELSDDA SYECQATEAALRSRRAKLTV LIPPEDTRIDGGPVILLQAG TPHNLTCRAFNAKPAATIIW FRDGTQQEGAVASTELLKDG KRETTVSQLLINPTDLDIGR VFTC |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 17-200 of human KIRREL (NP_060710.3). |
Additional Info
| Tissue Specificity | Abundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli. |
| Post Translational Modifications | Phosphorylation probably regulates the interaction with NSH2. Phosphorylated at Tyr-605 and Tyr-606 by FYN, leading to GRB2 binding. N-glycosylated. |
| Function | Required for proper function of the glomerular filtration barrier. It is involved in the maintenance of a stable podocyte architecture with interdigitating foot processes connected by specialized cell-cell junctions, known as the slit diaphragm. It is a signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1. |
| Protein Name | Kin Of Irre-Like Protein 1Kin Of Irregular Chiasm-Like Protein 1Nephrin-Like Protein 1 |
| Database Links | Reactome: R-HSA-373753 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinPredominantly Located At Podocyte Slit Diaphragm |
| Alternative Antibody Names | Anti-Kin Of Irre-Like Protein 1 antibodyAnti-Kin Of Irregular Chiasm-Like Protein 1 antibodyAnti-Nephrin-Like Protein 1 antibodyAnti-KIRREL1 antibodyAnti-KIRREL antibodyAnti-NEPH1 antibody |
Information sourced from Uniprot.org