| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DNA helicase B is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HELB |
| Gene ID: |
92797 |
| Uniprot ID: |
HELB_HUMAN |
| Specificity: |
This antibody detects endogenous levels of HELB at Human, Mouse |
| Immunogen: |
Synthesized peptide derived from human HELB |
| Post Translational Modifications | Phosphorylated at Ser-967 by CDK2 during the G1/S transition, resulting in its nuclear export into the cytoplasm. As S phase progresses, its exclusion from the nucleus promotes the activation of long-range resection. |
| Function | 5'-3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection. Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB. As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following phosphorylation. Acts independently of TP53BP1. Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates. During S phase, may facilitate cellular recovery from replication stress. |
| Protein Name | Dna Helicase BHdhb |
| Cellular Localisation | NucleusCytoplasmChromosomePredominantly NuclearPhosphorylation At Ser-967 By Cdk2 During The G1/S Transition Results In Its Nuclear Export Into The Cytoplasm As Cells Approach And Progress Through S PhaseFollowing Dna DamageRecruited To Sites Of Double-Strand Breaks By The Rpa ComplexRecruited To Chromatin Following Dna Damage Induced By Uv IrradiationOr Camptothecin Or Hydroxyurea Treatment |
| Alternative Antibody Names | Anti-Dna Helicase B antibodyAnti-Hdhb antibodyAnti-HELB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
