Anti-HELB antibody (STJA0009602)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human HELB
General Information
| Short Description | Rabbit polyclonal anti-DNA helicase B for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HELB |
| Gene ID | 92797 |
| Uniprot ID | HELB_HUMAN |
| Immunogen | Synthesized peptide derived from human HELB |
| Specificity | This antibody detects endogenous levels of HELB at Human, Mouse |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-967 by CDK2 during the G1/S transition, resulting in its nuclear export into the cytoplasm. As S phase progresses, its exclusion from the nucleus promotes the activation of long-range resection. |
| Function | 5'-3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection. Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB. As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following phosphorylation. Acts independently of TP53BP1. Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates. During S phase, may facilitate cellular recovery from replication stress. |
| Protein Name | Dna Helicase BHdhb |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmChromosomePredominantly NuclearPhosphorylation At Ser-967 By Cdk2 During The G1/S Transition Results In Its Nuclear Export Into The Cytoplasm As Cells Approach And Progress Through S PhaseFollowing Dna DamageRecruited To Sites Of Double-Strand Breaks By The Rpa ComplexRecruited To Chromatin Following Dna Damage Induced By Uv IrradiationOr Camptothecin Or Hydroxyurea Treatment |
| Alternative Antibody Names | Anti-Dna Helicase B antibodyAnti-Hdhb antibodyAnti-HELB antibody |
Information sourced from Uniprot.org