| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-HCAR3 (300-392) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HCAR3 |
| Gene ID: |
8843 |
| Uniprot ID: |
HCAR3_HUMAN |
| Immunogen Region: |
300-392 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 300-392 of human HCAR3 (NP_006009.2). |
| Immunogen Sequence: |
SFPNFFSTLINRCLQRKITG EPDNNRSTSVELTGDPNKTR GAPEALIANSGEPWSPSYLG PTSNNHSKKGHCHQEPASLE KQLGCCIE |
| Tissue Specificity | Expression largely restricted to adipose tissue and spleen. |
| Function | Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. |
| Protein Name | Hydroxycarboxylic Acid Receptor 3G-Protein Coupled Receptor 109bG-Protein Coupled Receptor Hm74G-Protein Coupled Receptor Hm74bNiacin Receptor 2Nicotinic Acid Receptor 2 |
| Database Links | Reactome: R-HSA-3296197Reactome: R-HSA-418594 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Hydroxycarboxylic Acid Receptor 3 antibodyAnti-G-Protein Coupled Receptor 109b antibodyAnti-G-Protein Coupled Receptor Hm74 antibodyAnti-G-Protein Coupled Receptor Hm74b antibodyAnti-Niacin Receptor 2 antibodyAnti-Nicotinic Acid Receptor 2 antibodyAnti-HCAR3 antibodyAnti-GPR109B antibodyAnti-HCA3 antibodyAnti-HM74B antibodyAnti-NIACR2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and SEPHS1 knockout (KO) 293T cells, using [KO Validated] SEPHS1 Rabbit polyclonal antibody (STJ11100796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89732/358210/STJ11100796_1__05587.1713122533.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SEPHS1 knockout (KO) 293T cells, using [KO Validated] SEPHS1 Rabbit polyclonal antibody (STJ11100796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
