Anti-HACE1 antibody (1-100) (STJ117867)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HACE1 (1-100) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HACE1 |
| Gene ID | 57531 |
| Uniprot ID | HACE1_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MERAMEQLNRLTRSLRRART VELPEDNETAVYTLMPMVMA DQHRSVSELLSNSKFDVNYA FGRVKRSLLHIAANCGSVEC LVLLLKKGANPNYQDISGCT |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HACE1 (NP_065822.2). |
Additional Info
| Tissue Specificity | Expressed in multiple tissues including heart, brain and kidney. |
| Post Translational Modifications | Autoubiquitinated. |
| Function | E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically binds GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB. |
| Protein Name | E3 Ubiquitin-Protein Ligase Hace1Hect Domain And Ankyrin Repeat-Containing E3 Ubiquitin-Protein Ligase 1Hect-Type E3 Ubiquitin Transferase Hace1 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | Golgi ApparatusGolgi Stack MembraneCytoplasmEndoplasmic ReticulumA Significant Portion Localizes To The Endoplasmic ReticulumTargeted To Golgi Membrane Via Its Interaction With Rab Proteins |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Hace1 antibodyAnti-Hect Domain And Ankyrin Repeat-Containing E3 Ubiquitin-Protein Ligase 1 antibodyAnti-Hect-Type E3 Ubiquitin Transferase Hace1 antibodyAnti-HACE1 antibodyAnti-KIAA1320 antibody |
Information sourced from Uniprot.org