• Western blot analysis of extracts of various cell lines, using HACE1 antibody (STJ117867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of HACE1 in paraffin-embedded rat testis using HACE1 Rabbit polyclonal antibody (STJ117867) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human placenta using HACE1 antibody (STJ117867) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of HACE1 in paraffin-embedded human placenta using HACE1 Rabbit polyclonal antibody (STJ117867) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat testis using HACE1 antibody (STJ117867) at dilution of 1:100 (40x lens).
  • Western blot analysis of various lysates using HACE1 Rabbit polyclonal antibody (STJ117867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HACE1 antibody (1-100) (STJ117867)

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STJ117867

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HACE1 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HACE1
Gene ID: 57531
Uniprot ID: HACE1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HACE1 (NP_065822.2).
Immunogen Sequence: MERAMEQLNRLTRSLRRART VELPEDNETAVYTLMPMVMA DQHRSVSELLSNSKFDVNYA FGRVKRSLLHIAANCGSVEC LVLLLKKGANPNYQDISGCT
Tissue Specificity Expressed in multiple tissues including heart, brain and kidney.
Function E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.
Protein Name E3 Ubiquitin-Protein Ligase Hace1
Hect Domain And Ankyrin Repeat-Containing E3 Ubiquitin-Protein Ligase 1
Hect-Type E3 Ubiquitin Transferase Hace1
Database Links Reactome: R-HSA-983168
Cellular Localisation Golgi Apparatus
Golgi Stack Membrane
Cytoplasm
Endoplasmic Reticulum
A Significant Portion Localizes To The Endoplasmic Reticulum
Targeted To Golgi Membrane Via Its Interaction With Rab Proteins
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Hace1 antibody
Anti-Hect Domain And Ankyrin Repeat-Containing E3 Ubiquitin-Protein Ligase 1 antibody
Anti-Hect-Type E3 Ubiquitin Transferase Hace1 antibody
Anti-HACE1 antibody
Anti-KIAA1320 antibody

Information sourced from Uniprot.org

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