| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GCK (250-465) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GCK |
| Gene ID: |
2645 |
| Uniprot ID: |
HXK4_HUMAN |
| Immunogen Region: |
250-465 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 250-465 of human Glucokinase (Glucokinase (GCK) ) (NP_000153.1). |
| Immunogen Sequence: |
RMCVNTEWGAFGDSGELDEF LLEYDRLVDESSANPGQQLY EKLIGGKYMGELVRLVLLRL VDENLLFHGEASEQLRTRGA FETRFVSQVESDTGDRKQIY NILSTLGLRPSTTDCDIVRR ACESVSTRAAHMCSAGLAGV INRMRESRSEDVMRITVGVD GSVYKLHPSFKERFHASVRR LTPSCEITFIESEEGSGRGA ALVSAVACKKACMLGQ |
| Function | Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant. Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues. Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell. In pancreas, plays an important role in modulating insulin secretion. In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage. Required to provide D-glucose 6-phosphate for the synthesis of glycogen. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. |
| Protein Name | Hexokinase-4Hk4GlucokinaseHexokinase Type IvHk IvHexokinase-D |
| Database Links | Reactome: R-HSA-170822Reactome: R-HSA-210745Reactome: R-HSA-5619073Reactome: R-HSA-5619107Reactome: R-HSA-70171Reactome: R-HSA-9615017 |
| Cellular Localisation | CytoplasmNucleusMitochondrionUnder Low Glucose ConcentrationsGck Associates With Gckr And The Inactive Complex Is Recruited To The Hepatocyte Nucleus |
| Alternative Antibody Names | Anti-Hexokinase-4 antibodyAnti-Hk4 antibodyAnti-Glucokinase antibodyAnti-Hexokinase Type Iv antibodyAnti-Hk Iv antibodyAnti-Hexokinase-D antibodyAnti-GCK antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
