• Western blot analysis of various lysates, using Ferritin Light Chain antibody (STJ11101302) at 1:8000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates, using Ferritin Light Chain antibody (STJ11101302) at 1:8000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded human thyroid tissue using Ferritin Light Chain rabbit monoclonal antibody (STJ11101302) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded human tonsil tissue using Ferritin Light Chain rabbit monoclonal antibody (STJ11101302) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded mouse spleen tissue using Ferritin Light Chain rabbit monoclonal antibody (STJ11101302) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded rat kidney tissue using Ferritin Light Chain rabbit monoclonal antibody (STJ11101302) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-FTL antibody (1-175) [S2MR] (STJ11101302)

SKU:
STJ11101302

£146.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:9000
IHC-P:1:400-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FTL
Gene ID: 2512
Uniprot ID: FRIL_HUMAN
Immunogen Region: 1-175
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human Ferritin Light Chain (NP_000137.2).
Immunogen Sequence: MSSQIRQNYSTDVEAAVNSL VNLYLQASYTYLSLGFYFDR DDVALEGVSHFFRELAEEKR EGYERLLKMQNQRGGRALFQ DIKKPAEDEWGKTPDAMKAA MALEKKLNQALLDLHALGSA RTDPHLCDFLETHFLDEEVK LIKKMGDHLTNLHRLGGPEA GLGEYLFERLTLKHD
Function Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron.
Protein Name Ferritin Light Chain
Ferritin L Subunit
Database Links Reactome: R-HSA-3000480
Reactome: R-HSA-432722
Reactome: R-HSA-6798695
Reactome: R-HSA-917937
Cellular Localisation Cytoplasmic Vesicle
Autophagosome
Cytoplasm
Autolysosome
Alternative Antibody Names Anti-Ferritin Light Chain antibody
Anti-Ferritin L Subunit antibody
Anti-FTL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance