• Western blot analysis of various lysates, using Ferritin Light Chain antibody (STJ11101302) at 1:8000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates, using Ferritin Light Chain antibody (STJ11101302) at 1:8000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded human thyroid tissue using Ferritin Light Chain rabbit monoclonal antibody (STJ11101302) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded human tonsil tissue using Ferritin Light Chain rabbit monoclonal antibody (STJ11101302) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded mouse spleen tissue using Ferritin Light Chain rabbit monoclonal antibody (STJ11101302) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded rat kidney tissue using Ferritin Light Chain rabbit monoclonal antibody (STJ11101302) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-FTL antibody (1-175) [S2MR] (STJ11101302)

SKU:
STJ11101302

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Ferritin Light Chain (1-175) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:9000
IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FTL
Gene ID: 2512
Uniprot ID: FRIL_HUMAN
Immunogen Region: 1-175
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human Ferritin Light Chain (NP_000137.2).
Immunogen Sequence: MSSQIRQNYSTDVEAAVNSL VNLYLQASYTYLSLGFYFDR DDVALEGVSHFFRELAEEKR EGYERLLKMQNQRGGRALFQ DIKKPAEDEWGKTPDAMKAA MALEKKLNQALLDLHALGSA RTDPHLCDFLETHFLDEEVK LIKKMGDHLTNLHRLGGPEA GLGEYLFERLTLKHD
Function Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
Protein Name Ferritin Light Chain
Ferritin L Subunit
Database Links Reactome: R-HSA-3000480
Reactome: R-HSA-432722
Reactome: R-HSA-6798695
Reactome: R-HSA-917937
Alternative Antibody Names Anti-Ferritin Light Chain antibody
Anti-Ferritin L Subunit antibody
Anti-FTL antibody

Information sourced from Uniprot.org

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