• Immunofluorescence analysis of L929 cells using EXT2 Rabbit polyclonal antibody (STJ111122) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using EXT2 antibody (STJ111122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EXT2 antibody (80-340) (STJ111122)

SKU:
STJ111122

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EXT2 (80-340) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EXT2
Gene ID: 2132
Uniprot ID: EXT2_HUMAN
Immunogen Region: 80-340
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EXT2 (NP_000392.3).
Immunogen Sequence: PHSIESSNDWNVEKRSIRDV PVVRLPADSPIPERGDLSCR MHTCFDVYRCGFNPKNKIKV YIYALKKYVDDFGVSVSNTI SREYNELLMAISDSDYYTDD INRACLFVPSIDVLNQNTLR IKETAQAMAQLSRWDRGTNH LLFNMLPGGPPDYNTALDVP RDRALLAGGGFSTWTYRQGY DVSIPVYSPLSAEVDLPEKG PGPRQYFLLSSQVGLHPEYR EDLEALQVKHGESVLVLDK
Tissue Specificity Ubiquitous.
Function Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Required for the exosomal release of SDCBP, CD63 and syndecan.
Protein Name Exostosin-2
Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan/N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-Acetylglucosaminyltransferase
Multiple Exostoses Protein 2
Putative Tumor Suppressor Protein Ext2
Database Links Reactome: R-HSA-2022928
Reactome: R-HSA-3656237
Reactome: R-HSA-3656253
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Golgi Apparatus Membrane
Golgi Apparatus
Cis-Golgi Network Membrane
The Ext1/Ext2 Complex Is Localized In The Golgi Apparatus
Alternative Antibody Names Anti-Exostosin-2 antibody
Anti-Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan/N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-Acetylglucosaminyltransferase antibody
Anti-Multiple Exostoses Protein 2 antibody
Anti-Putative Tumor Suppressor Protein Ext2 antibody
Anti-EXT2 antibody

Information sourced from Uniprot.org

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