Anti-EXT2 antibody (80-340) (STJ111122)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EXT2 (80-340) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EXT2 |
| Gene ID | 2132 |
| Uniprot ID | EXT2_HUMAN |
| Immunogen Region | 80-340 |
| Immunogen Sequence | PHSIESSNDWNVEKRSIRDV PVVRLPADSPIPERGDLSCR MHTCFDVYRCGFNPKNKIKV YIYALKKYVDDFGVSVSNTI SREYNELLMAISDSDYYTDD INRACLFVPSIDVLNQNTLR IKETAQAMAQLSRWDRGTNH LLFNMLPGGPPDYNTALDVP RDRALLAGGGFSTWTYRQGY DVSIPVYSPLSAEVDLPEKG PGPRQYFLLSSQVGLHPEYR EDLEALQVKHGESVLVLDK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EXT2 (NP_000392.3). |
Additional Info
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | N-glycosylated at Asn-637. A soluble form is generated by proteolytic processing. |
| Function | Glycosyltransferase forming with EXT1 the heterodimeric heparan sulfate polymerase which catalyzes the elongation of the heparan sulfate glycan backbone. Glycan backbone extension consists in the alternating transfer of (1->4)-beta-D-GlcA and (1->4)-alpha-D-GlcNAc residues from their respective UDP-sugar donors. Both EXT1 and EXT2 are required for the full activity of the polymerase since EXT1 bears the N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity within the complex while EXT2 carries the glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity. Heparan sulfate proteoglycans are ubiquitous components of the extracellular matrix and play an important role in tissue homeostasis and signaling. |
| Protein Name | Exostosin-2Exostosin Glycosyltransferase 2Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-AcetylglucosaminyltransferaseHeparan Sulfate Co-Polymerase Subunit Ext1Multiple Exostoses Protein 2 |
| Database Links | Reactome: R-HSA-2022928Reactome: R-HSA-3656237Reactome: R-HSA-3656253 |
| Cellular Localisation | Golgi Apparatus MembraneSingle-Pass Type Ii Membrane ProteinGolgi ApparatusCis-Golgi Network MembraneEndoplasmic Reticulum MembraneSecretedThe Active Heparan Sulfate Polymerase Complex Composed Of Ext1 And Ext2 Is Localized To The Golgi ApparatusIf Both Proteins Are Individually Detected In The Endoplasmic ReticulumThe Formation Of The Complex Promotes Their Transport To The Golgi |
| Alternative Antibody Names | Anti-Exostosin-2 antibodyAnti-Exostosin Glycosyltransferase 2 antibodyAnti-Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-Acetylglucosaminyltransferase antibodyAnti-Heparan Sulfate Co-Polymerase Subunit Ext1 antibodyAnti-Multiple Exostoses Protein 2 antibodyAnti-EXT2 antibody |
Information sourced from Uniprot.org