| Short Description : | Rabbit polyclonal EML4 (360-560) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EML4 |
| Gene ID: | 27436 |
| Uniprot ID: | EMAL4_HUMAN |
| Immunogen Region: | 360-560 |
| Immunogen Sequence: | GTFERGVGCLDFSKADSGVH LCVIDDSNEHMLTVWDWQKK AKGAEIKTTNEVVLAVEFHP TDANTIITCGKSHIFFWTWS GNSLTRKQGIFGKYEKPKFV QCLAFLGNGDVLTGDSGGVM LIWSKTTVEPTPGKGPKGVY QISKQIKAHDGSVFTLCQMR NGMLLTGGGKDRKIILWDHD LNPEREIEVPDQYGTIRAVA E |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 360-560 of human EML4 (NP_061936.2). |
| Post Translational Modifications | Phosphorylated during mitosis. Phosphorylation at Ser-144 and Ser-146 promotes its dissociation from microtubules during mitosis which is required for efficient chromosome congression. |
| Function | Essential for the formation and stability of microtubules (MTs). Required for the organization of the mitotic spindle and for the proper attachment of kinetochores to MTs. Promotes the recruitment of NUDC to the mitotic spindle for mitotic progression. |
| Protein Name | Echinoderm Microtubule-Associated Protein-Like 4Emap-4Restrictedly Overexpressed Proliferation-Associated ProteinRopp 120 |
| Database Links | Reactome: R-HSA-9648025Reactome: R-HSA-9700645Reactome: R-HSA-9725370 |
| Cellular Localisation | CytoplasmCytoskeletonSpindleMicrotubule Organizing CenterMidbodyLocalizes To Microtubules (Mts) During Interphase With A Significantly Reduced Affinity For Mts During Mitosis |
| Alternative Antibody Names | Anti-Echinoderm Microtubule-Associated Protein-Like 4 antibodyAnti-Emap-4 antibodyAnti-Restrictedly Overexpressed Proliferation-Associated Protein antibodyAnti-Ropp 120 antibodyAnti-EML4 antibodyAnti-C2orf2 antibodyAnti-EMAPL4 antibody |
Information sourced from Uniprot.org
