• Western blot analysis of extracts of various cell lines, using eIF2B epsilon (EIF2B5) (EIF2B5) rabbit monoclonal antibody (STJ11102350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Confocal imaging of MCF7 cells using eIF2B epsilon (EIF2B5) Rabbit monoclonal antibody (STJ11102350, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded rat brain using eIF2B epsilon (EIF2B5) (EIF2B5) rabbit monoclonal antibody (STJ11102350) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human breast cancer tissue using eIF2B epsilon (EIF2B5) Rabbit monoclonal antibody (STJ11102350) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human lung cancer using eIF2B epsilon (EIF2B5) (EIF2B5) rabbit monoclonal antibody (STJ11102350) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human colon tissue using eIF2B epsilon (EIF2B5) Rabbit monoclonal antibody (STJ11102350) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using eIF2B epsilon (EIF2B5) (EIF2B5) rabbit monoclonal antibody (STJ11102350) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human breast tissue using eIF2B epsilon (EIF2B5) Rabbit monoclonal antibody (STJ11102350) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of NIH-3T3 cells using eIF2B epsilon (EIF2B5) (EIF2B5) rabbit monoclonal antibody (STJ11102350) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human tonsil tissue using eIF2B epsilon (EIF2B5) Rabbit monoclonal antibody (STJ11102350) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of U-2 OS cells using eIF2B epsilon (EIF2B5) (EIF2B5) rabbit monoclonal antibody (STJ11102350) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using eIF2B epsilon (EIF2B5) (EIF2B5) Rabbit monoclonal antibody (STJ11102350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EIF2B5 antibody (1-100) [S0MR] (STJ11102350)

SKU:
STJ11102350

$174.83 - $775.58
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2B5
Gene ID: 8893
Uniprot ID: EI2BE_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF2B epsilon (EIF2B5) (EIF2B5) (Q13144).
Immunogen Sequence: MAAPVVAPPGVVVSRANKRS GAGPGGSGGGGARGAEEEPP PPLQAVLVADSFDRRFFPIS KDQPRVLLPLANVALIDYTL EFLTATGVQETFVFCCWKAA
Post Translational Modifications Phosphorylated at Ser-544 by DYRK2.this is required for subsequent phosphorylation by GSK3B. Phosphorylated on serine and threonine residues by GSK3B.phosphorylation inhibits its function. Polyubiquitinated, probably by NEDD4.
Function Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.
Protein Name Translation Initiation Factor Eif2b Subunit Epsilon
Eif2b Gdp-Gtp Exchange Factor Subunit Epsilon
Database Links Reactome: R-HSA-72731
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Translation Initiation Factor Eif2b Subunit Epsilon antibody
Anti-Eif2b Gdp-Gtp Exchange Factor Subunit Epsilon antibody
Anti-EIF2B5 antibody
Anti-EIF2BE antibody

Information sourced from Uniprot.org

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