| Short Description : | Rabbit monoclonal eIF2B epsilon (1-100) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S0MR |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EIF2B5 |
| Gene ID: | 8893 |
| Uniprot ID: | EI2BE_HUMAN |
| Immunogen Region: | 1-100 |
| Immunogen Sequence: | MAAPVVAPPGVVVSRANKRS GAGPGGSGGGGARGAEEEPP PPLQAVLVADSFDRRFFPIS KDQPRVLLPLANVALIDYTL EFLTATGVQETFVFCCWKAA |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF2B epsilon (EIF2B5) (EIF2B5) (Q13144). |
| Post Translational Modifications | Phosphorylated at Ser-544 by DYRK2.this is required for subsequent phosphorylation by GSK3B. Phosphorylated on serine and threonine residues by GSK3B.phosphorylation inhibits its function. Polyubiquitinated, probably by NEDD4. |
| Function | Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed. |
| Protein Name | Translation Initiation Factor Eif2b Subunit EpsilonEif2b Gdp-Gtp Exchange Factor Subunit Epsilon |
| Database Links | Reactome: R-HSA-72731 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Translation Initiation Factor Eif2b Subunit Epsilon antibodyAnti-Eif2b Gdp-Gtp Exchange Factor Subunit Epsilon antibodyAnti-EIF2B5 antibodyAnti-EIF2BE antibody |
Information sourced from Uniprot.org
