• Western blot analysis of extracts of various cell lines, using eIF2B epsilon (EIF2B5) (EIF2B5) rabbit monoclonal antibody (STJ11102350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Confocal imaging of MCF7 cells using eIF2B epsilon (EIF2B5) Rabbit monoclonal antibody (STJ11102350, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded rat brain using eIF2B epsilon (EIF2B5) (EIF2B5) rabbit monoclonal antibody (STJ11102350) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human breast cancer tissue using eIF2B epsilon (EIF2B5) Rabbit monoclonal antibody (STJ11102350) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human lung cancer using eIF2B epsilon (EIF2B5) (EIF2B5) rabbit monoclonal antibody (STJ11102350) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human colon tissue using eIF2B epsilon (EIF2B5) Rabbit monoclonal antibody (STJ11102350) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using eIF2B epsilon (EIF2B5) (EIF2B5) rabbit monoclonal antibody (STJ11102350) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human breast tissue using eIF2B epsilon (EIF2B5) Rabbit monoclonal antibody (STJ11102350) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of NIH-3T3 cells using eIF2B epsilon (EIF2B5) (EIF2B5) rabbit monoclonal antibody (STJ11102350) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human tonsil tissue using eIF2B epsilon (EIF2B5) Rabbit monoclonal antibody (STJ11102350) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of U-2 OS cells using eIF2B epsilon (EIF2B5) (EIF2B5) rabbit monoclonal antibody (STJ11102350) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using eIF2B epsilon (EIF2B5) (EIF2B5) Rabbit monoclonal antibody (STJ11102350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EIF2B5 antibody (1-100) [S0MR] (STJ11102350)

SKU:
STJ11102350

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-eIF2B epsilon (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2B5
Gene ID: 8893
Uniprot ID: EI2BE_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF2B epsilon (EIF2B5) (EIF2B5) (Q13144).
Immunogen Sequence: MAAPVVAPPGVVVSRANKRS GAGPGGSGGGGARGAEEEPP PPLQAVLVADSFDRRFFPIS KDQPRVLLPLANVALIDYTL EFLTATGVQETFVFCCWKAA
Post Translational Modifications Phosphorylated at Ser-544 by DYRK2.this is required for subsequent phosphorylation by GSK3B. Phosphorylated on serine and threonine residues by GSK3B.phosphorylation inhibits its function. Polyubiquitinated, probably by NEDD4.
Function Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Protein Name Translation Initiation Factor Eif-2b Subunit Epsilon
Eif-2b Gdp-Gtp Exchange Factor Subunit Epsilon
Database Links Reactome: R-HSA-72731
Alternative Antibody Names Anti-Translation Initiation Factor Eif-2b Subunit Epsilon antibody
Anti-Eif-2b Gdp-Gtp Exchange Factor Subunit Epsilon antibody
Anti-EIF2B5 antibody
Anti-EIF2BE antibody

Information sourced from Uniprot.org

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