Anti-ECD antibody (435-644) (STJ11104764)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Protein Ecdysoneless Homolog (435-644) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ECD |
| Gene ID | 11319 |
| Uniprot ID | ECD_HUMAN |
| Immunogen Region | 435-644 |
| Immunogen Sequence | ESESVSKEEKEQNYDLTEVS ESMKAFISKVSTHKGAELPR EPSEAPITFDADSFLNYFDK ILGPRPNESDSDDLDDEDFE CLDSDDDLDFETHEPGEEAS LKGTLDNLKSYMAQMDQELA HTCISKSFTTRNQVEPVSQT TDNNSDEEDSGTGESVMAPV DVDLNLVSNILESYSSQAGL AGPASNLLQSMGVQLPDNTD HRPTSKPTKN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 435-644 of human ECD (NP_009196.1). |
Additional Info
| Tissue Specificity | Highly expressed in muscle and heart. Over-expressed in pancreatic and breast cancers. |
| Post Translational Modifications | Phosphorylated predominantly by CK2 on two serine-containing clusters.involved in cell cycle regulation activity. |
| Function | Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing. Participates together with DDX39A in mRNA nuclear export. |
| Protein Name | Protein Ecdysoneless HomologHuman Suppressor Of Gcr TwoHsgt1 |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusPredominantly Is Located In The Cytoplasm |
| Alternative Antibody Names | Anti-Protein Ecdysoneless Homolog antibodyAnti-Human Suppressor Of Gcr Two antibodyAnti-Hsgt1 antibodyAnti-ECD antibody |
Information sourced from Uniprot.org