• Western blot analysis of various lysates, using ECD antibody (STJ11104764) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of HeLa, using ECD antibody (STJ11104764) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ECD antibody (435-644) (STJ11104764)

SKU:
STJ11104764

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein Ecdysoneless Homolog (435-644) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ECD
Gene ID: 11319
Uniprot ID: ECD_HUMAN
Immunogen Region: 435-644
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 435-644 of human ECD (NP_009196.1).
Immunogen Sequence: ESESVSKEEKEQNYDLTEVS ESMKAFISKVSTHKGAELPR EPSEAPITFDADSFLNYFDK ILGPRPNESDSDDLDDEDFE CLDSDDDLDFETHEPGEEAS LKGTLDNLKSYMAQMDQELA HTCISKSFTTRNQVEPVSQT TDNNSDEEDSGTGESVMAPV DVDLNLVSNILESYSSQAGL AGPASNLLQSMGVQLPDNTD HRPTSKPTKN
Tissue Specificity Highly expressed in muscle and heart. Over-expressed in pancreatic and breast cancers.
Post Translational Modifications Phosphorylated predominantly by CK2 on two serine-containing clusters.involved in cell cycle regulation activity.
Function Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.
Protein Name Protein Ecdysoneless Homolog
Human Suppressor Of Gcr Two
Hsgt1
Cellular Localisation Cytoplasm
Nucleus
Predominantly Is Located In The Cytoplasm
Alternative Antibody Names Anti-Protein Ecdysoneless Homolog antibody
Anti-Human Suppressor Of Gcr Two antibody
Anti-Hsgt1 antibody
Anti-ECD antibody

Information sourced from Uniprot.org

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