Anti-DEAD-box protein 6 antibody (C-Term) (STJ70871)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70871-100
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General Information

Short DescriptionGoat polyclonal antibody anti-DEAD-box protein 6 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
ApplicationsPep-ELISA/WB/IHC
HostGoat
ReactivityHuman/Mouse/Rat/Dog
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 50kDa band observed in lysates of cell lines Daudi, Jurkat and HepG2 (calculated MW of 53.2kDa according to NP_004388.1). Recommended concentration: 0.3-1ยตg/ml. Primary incubat
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolDDX6
Gene ID1656
Uniprot IDDDX6_HUMAN
Accession NumberNP_004388.1
Immunogen RegionC-Term
Immunogen SequenceAEYHSEPVEDEKP

Additional Info

Post Translational Modifications Sumoylated.
Function Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions. Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation. In the process of mRNA degradation, plays a role in mRNA decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.
Protein Name Probable Atp-Dependent Rna Helicase Ddx6
Atp-Dependent Rna Helicase P54
Dead Box Protein 6
Oncogene Rck
Database Links Reactome: R-HSA-430039
Cellular Localisation Cytoplasm
P-Body
Nucleus
Cytoplasmic Ribonucleoprotein Granule
Imported In The Nucleus Via Interaction With Eif4enif1/4e-T Via A Piggy-Back Mechanism
Upon Cellular Stress
Relocalizes To Stress Granules
Alternative Antibody Names Anti-Probable Atp-Dependent Rna Helicase Ddx6 antibody
Anti-Atp-Dependent Rna Helicase P54 antibody
Anti-Dead Box Protein 6 antibody
Anti-Oncogene Rck antibody
Anti-DDX6 antibody
Anti-HLR2 antibody
Anti-RCK antibody

Information sourced from Uniprot.org

Citations

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