| Short Description : | Rabbit polyclonal DAP (1-102) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:20-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DAP |
| Gene ID: | 1611 |
| Uniprot ID: | DAP1_HUMAN |
| Immunogen Region: | 1-102 |
| Immunogen Sequence: | MSSPPEGKLETKAGHPPAVK AGGMRIVQKHPHTGDTKEEK DKDDQEWESPSPPKPTVFIS GVIARGDKDFPPAAAQVAHQ KPHASMDKHPSPRTQHIQQP RK |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human DAP (NP_004385.1). |
| Post Translational Modifications | Phosphorylated. Phosphorylation by MTOR inhibits the suppressive activity of DAP toward autophagy. |
| Function | Ribosome-binding protein involved in ribosome hibernation, a process during which ribosomes are stabilized in an inactive state and preserved from proteasomal degradation. Acts via its association with eiF5a (EIF5A and EIF5A2) at the polypeptide exit tunnel of the ribosome, preventing mRNA translation. Involved in ribosome hibernation in the mature oocyte by preventing mRNA translation, leading to ribosome inactivation. Ribosomes, which are produced in large quantities during oogenesis, are stored and translationally repressed in the oocyte and early embryo. Also acts as a negative regulator of autophagy. Involved in mediating interferon-gamma-induced cell death. |
| Protein Name | Death-Associated Protein 1Dap-1 |
| Database Links | |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Death-Associated Protein 1 antibodyAnti-Dap-1 antibodyAnti-DAP antibodyAnti-DAP1 antibody |
Information sourced from Uniprot.org
