• Immunofluorescence analysis of MCF-7 cells using DAP antibody (STJ27903). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using DAP antibody (STJ27903) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using DAP antibody (STJ27903) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-DAP antibody (1-102) (STJ27903)

SKU:
STJ27903

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DAP (1-102) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DAP
Gene ID: 1611
Uniprot ID: DAP1_HUMAN
Immunogen Region: 1-102
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human DAP (NP_004385.1).
Immunogen Sequence: MSSPPEGKLETKAGHPPAVK AGGMRIVQKHPHTGDTKEEK DKDDQEWESPSPPKPTVFIS GVIARGDKDFPPAAAQVAHQ KPHASMDKHPSPRTQHIQQP RK
Post Translational Modifications Phosphorylated. Phosphorylation by MTOR inhibits the suppressive activity of DAP toward autophagy.
Function Ribosome-binding protein involved in ribosome hibernation, a process during which ribosomes are stabilized in an inactive state and preserved from proteasomal degradation. Acts via its association with eiF5a (EIF5A and EIF5A2) at the polypeptide exit tunnel of the ribosome, preventing mRNA translation. Involved in ribosome hibernation in the mature oocyte by preventing mRNA translation, leading to ribosome inactivation. Ribosomes, which are produced in large quantities during oogenesis, are stored and translationally repressed in the oocyte and early embryo. Also acts as a negative regulator of autophagy. Involved in mediating interferon-gamma-induced cell death.
Protein Name Death-Associated Protein 1
Dap-1
Alternative Antibody Names Anti-Death-Associated Protein 1 antibody
Anti-Dap-1 antibody
Anti-DAP antibody
Anti-DAP1 antibody

Information sourced from Uniprot.org

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