• Immunofluorescence analysis of L929 cells using CYTH1 Rabbit polyclonal antibody (STJ117546) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CYTH1 Rabbit polyclonal antibody (STJ117546) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CYTH1 antibody (1-70) (STJ117546)

SKU:
STJ117546

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CYTH1 (1-70) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYTH1
Gene ID: 9267
Uniprot ID: CYH1_HUMAN
Immunogen Region: 1-70
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human CYTH1 (NP_004753.1).
Immunogen Sequence: MEEDDSYVPSDLTAEERQEL ENIRRRKQELLADIQRLKDE IAEVANEIENLGSTEERKNM QRNKQVAMGR
Tissue Specificity Ubiquitous.
Post Translational Modifications Ubiquitinated by SCF(FBXW11) E3 ubiquitin-protein ligase complex. Ubiquitination induces proteasomal degradation.
Function Promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization, through regulation of ARF6 activity.
Protein Name Cytohesin-1
Ph - Sec7 And Coiled-Coil Domain-Containing Protein 1
Sec7 Homolog B2-1
Database Links Reactome: R-HSA-6811438
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasm
Cytosol
Cell Junction
Tight Junction
Adherens Junction
Colocalized With Tjp1 During Epithelial Polarization
Alternative Antibody Names Anti-Cytohesin-1 antibody
Anti-Ph - Sec7 And Coiled-Coil Domain-Containing Protein 1 antibody
Anti-Sec7 Homolog B2-1 antibody
Anti-CYTH1 antibody
Anti-D17S811E antibody
Anti-PSCD1 antibody

Information sourced from Uniprot.org

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