| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CYP2R1 |
| Gene ID: | 120227 |
| Uniprot ID: | CP2R1_HUMAN |
| Immunogen Region: | 252-501 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 252-501 of human CYP2R1 (NP_078790.2). |
| Immunogen Sequence: | VVYDFLSRLIEKASVNRKPQ LPQHFVDAYLDEMDQGKNDP SSTFSKENLIFSVGELIIAG TETTTNVLRWAILFMALYPN IQGQVQKEIDLIMGPNGKPS WDDKCKMPYTEAVLHEVLRF CNIVPLGIFHATSEDAVVRG YSIPKGTTVITNLYSVHFDE KYWRDPEVFHPERFLDSSGY FAKKEALVPFSLGRRHCLGE HLARMEMFLFFTALLQRFHL HFPHELVPDLKPRLGMTLQ |
| Function | A cytochrome P450 monooxygenase involved in activation of vitamin D precursors. Catalyzes hydroxylation at C-25 of both forms of vitamin D, vitamin D(2) and D(3) (calciol). Can metabolize vitamin D analogs/prodrugs 1alpha-hydroxyvitamin D(2) (doxercalciferol) and 1alpha-hydroxyvitamin D(3) (alfacalcidol) forming 25-hydroxy derivatives. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). |
| Protein Name | Vitamin D 25-HydroxylaseCytochrome P450 2r1 |
| Database Links | Reactome: R-HSA-196791Reactome: R-HSA-211916Reactome: R-HSA-5579027 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinMicrosome Membrane |
| Alternative Antibody Names | Anti-Vitamin D 25-Hydroxylase antibodyAnti-Cytochrome P450 2r1 antibodyAnti-CYP2R1 antibody |
Information sourced from Uniprot.org