| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CYP2R1 (252-501) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CYP2R1 |
| Gene ID: |
120227 |
| Uniprot ID: |
CP2R1_HUMAN |
| Immunogen Region: |
252-501 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 252-501 of human CYP2R1 (NP_078790.2). |
| Immunogen Sequence: |
VVYDFLSRLIEKASVNRKPQ LPQHFVDAYLDEMDQGKNDP SSTFSKENLIFSVGELIIAG TETTTNVLRWAILFMALYPN IQGQVQKEIDLIMGPNGKPS WDDKCKMPYTEAVLHEVLRF CNIVPLGIFHATSEDAVVRG YSIPKGTTVITNLYSVHFDE KYWRDPEVFHPERFLDSSGY FAKKEALVPFSLGRRHCLGE HLARMEMFLFFTALLQRFHL HFPHELVPDLKPRLGMTLQ |
| Function | A cytochrome P450 monooxygenase involved in activation of vitamin D precursors. Catalyzes hydroxylation at C-25 of both forms of vitamin D, vitamin D(2) and D(3) (calciol). Can metabolize vitamin D analogs/prodrugs 1alpha-hydroxyvitamin D(2) (doxercalciferol) and 1alpha-hydroxyvitamin D(3) (alfacalcidol) forming 25-hydroxy derivatives. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). |
| Protein Name | Vitamin D 25-HydroxylaseCytochrome P450 2r1 |
| Database Links | Reactome: R-HSA-196791Reactome: R-HSA-211916Reactome: R-HSA-5579027 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinMicrosome Membrane |
| Alternative Antibody Names | Anti-Vitamin D 25-Hydroxylase antibodyAnti-Cytochrome P450 2r1 antibodyAnti-CYP2R1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
