![Immunohistochemistry analysis of COX5A in paraffin-embedded human lung cancer tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178010/370088/STJ11105366_1__89142.1713136011.jpg?c=1 "Immunohistochemistry analysis of COX5A in paraffin-embedded human lung cancer tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of COX5A in paraffin-embedded human liver cancer tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178010/370089/STJ11105366_2__66220.1713136013.jpg?c=1 "Immunohistochemistry analysis of COX5A in paraffin-embedded human liver cancer tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of COX5A in paraffin-embedded mouse kidney tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178010/370090/STJ11105366_3__42604.1713136014.jpg?c=1 "Immunohistochemistry analysis of COX5A in paraffin-embedded mouse kidney tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of COX5A in paraffin-embedded human colon carcinoma tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178010/370091/STJ11105366_4__45239.1713136015.jpg?c=1 "Immunohistochemistry analysis of COX5A in paraffin-embedded human colon carcinoma tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of COX5A in paraffin-embedded rat brain tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178010/370092/STJ11105366_5__97603.1713136017.jpg?c=1 "Immunohistochemistry analysis of COX5A in paraffin-embedded rat brain tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Western blot analysis of lysates from wild type (WT) and COX5A knockdown (KD) HeLa (KD) cells, using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178010/370093/STJ11105366_6__26137.1713136017.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and COX5A knockdown (KD) HeLa (KD) cells, using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of various lysates, using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178010/370094/STJ11105366_7__73863.1713136018.jpg?c=1 "Western blot analysis of various lysates, using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry analysis of COX5A in paraffin-embedded human lung cancer tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/178010/370088/STJ11105366_1__89142.1713136011.jpg?c=1 "Immunohistochemistry analysis of COX5A in paraffin-embedded human lung cancer tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
Anti-COX5A antibody (1-150) (STJ11105366)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Cytochrome C Oxidase Subunit 5a-Mitochondrial (1-150) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | COX5A |
| Gene ID | 9377 |
| Uniprot ID | COX5A_HUMAN |
| Immunogen Region | 1-150 |
| Immunogen Sequence | MLGAALRRCAVAATTRADPR GLLHSARTPGPAVAIQSVRC YSHGSQETDEEFDARWVTYF NKPDIDAWELRKGINTLVTY DMVPEPKIIDAALRACRRLN DFASTVRILEVVKDKAGPHK EIYPYVIQELRPTLNELGIS TPEELGLDKV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human COX5A (NP_004246.2). |
Additional Info
| Post Translational Modifications | In response to mitochondrial stress, the precursor protein is ubiquitinated by the SIFI complex in the cytoplasm before mitochondrial import, leading to its degradation. Within the SIFI complex, UBR4 initiates ubiquitin chain that are further elongated or branched by KCMF1. |
| Function | Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. |
| Protein Name | Cytochrome C Oxidase Subunit 5a - MitochondrialCytochrome C Oxidase Polypeptide Va |
| Database Links | Reactome: R-HSA-5628897Reactome: R-HSA-611105Reactome: R-HSA-9707564Reactome: R-HSA-9837999Reactome: R-HSA-9864848 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinMatrix Side |
| Alternative Antibody Names | Anti-Cytochrome C Oxidase Subunit 5a - Mitochondrial antibodyAnti-Cytochrome C Oxidase Polypeptide Va antibodyAnti-COX5A antibody |
Information sourced from Uniprot.org