• Western blot analysis of extracts of various cell lines, using CHD3 rabbit monoclonal antibody (STJ11102316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of mouse brain, using CHD3 rabbit monoclonal antibody (STJ11102316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of Rat brain, using CHD3 rabbit monoclonal antibody (STJ11102316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of CHD3 in paraffin-embedded human cervix cancer tissue using CHD3 rabbit monoclonal antibody (STJ11102316) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CHD3 in paraffin-embedded human tonsil tissue using CHD3 rabbit monoclonal antibody (STJ11102316) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CHD3 in paraffin-embedded mouse brain tissue using CHD3 rabbit monoclonal antibody (STJ11102316) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CHD3 in paraffin-embedded mouse kidney tissue using CHD3 rabbit monoclonal antibody (STJ11102316) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CHD3 in paraffin-embedded rat spleen tissue using CHD3 rabbit monoclonal antibody (STJ11102316) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of NIH/3T3 cells using CHD3 rabbit monoclonal antibody (STJ11102316) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CHD3 antibody (1-100) [S6MR] (STJ11102316)

SKU:
STJ11102316

$286.88 - $677.03
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CHD3 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHD3
Gene ID: 1107
Uniprot ID: CHD3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CHD3 (Q12873).
Immunogen Sequence: MKAADTVILWARSKNDQLRI SFPPGLCWGDRMPDKDDIRL LPSALGVKKRKRGPKKQKEN KPGKPRKRKKRDSEEEFGSE RDEYREKSESGGSEYGTGPG
Tissue Specificity Widely expressed.
Post Translational Modifications Sumoylation at Lys-1971 results in dissociation from chromatin and suppression of transcriptional repression.
Function ATP-dependent helicase that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Involved in transcriptional repression as part of the NuRD complex. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
Protein Name Chromodomain-Helicase-Dna-Binding Protein 3
Chd-3
Atp-Dependent Helicase Chd3
Mi-2 Autoantigen 240 Kda Protein
Mi2-Alpha
Zinc Finger Helicase
Hzfh
Database Links Reactome: R-HSA-3214815
Reactome: R-HSA-427389
Reactome: R-HSA-4551638
Reactome: R-HSA-6804758
Reactome: R-HSA-73762
Reactome: R-HSA-8943724
Reactome: R-HSA-9679191
Cellular Localisation Nucleus
Pml Body
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Associates With Centrosomes In Interphase And Mitosis
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-Chromodomain-Helicase-Dna-Binding Protein 3 antibody
Anti-Chd-3 antibody
Anti-Atp-Dependent Helicase Chd3 antibody
Anti-Mi-2 Autoantigen 240 Kda Protein antibody
Anti-Mi2-Alpha antibody
Anti-Zinc Finger Helicase antibody
Anti-Hzfh antibody
Anti-CHD3 antibody

Information sourced from Uniprot.org

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