| Host: |
Goat |
| Applications: |
Pep-ELISA/IHC |
| Reactivity: |
Human/Mouse/Rat/Dog/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-Cdc42-binding kinase alpha (N-Term) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
IHC-3.75µg/mlELISA-antibody detection limit dilution 1:16000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
CDC42BPA |
| Gene ID: |
8476 |
| Uniprot ID: |
MRCKA_HUMAN |
| Immunogen Region: |
N-Term |
| Accession Number: |
NP_055641.3; NP_003598.2 |
| Specificity: |
This antibody is expected to recognise both reported Human isoforms (NP_055641.3 and NP_003598.2). |
| Immunogen Sequence: |
SGEVRLRQLEQFI |
| Post Translational Modifications | Proteolytically cleaved by caspases upon apoptosis induction. The cleavage at Asp-478 by CASP3 increases its kinase activity (in vitro). |
| Function | Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake. In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation. Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells. |
| Protein Name | Serine/Threonine-Protein Kinase Mrck AlphaCdc42-Binding Protein Kinase AlphaDmpk-Like AlphaMyotonic Dystrophy Kinase-Related Cdc42-Binding Kinase AlphaMrck AlphaMyotonic Dystrophy Protein Kinase-Like Alpha |
| Database Links | Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013406Reactome: R-HSA-9013409 |
| Cellular Localisation | CytoplasmCell ProjectionLamellipodiumDisplays A Dispersed Punctate Distribution And Concentrates Along The Cell PeripheryEspecially At The Leading Edge And Cell-Cell JunctionThis Concentration Is Ph-Domain DependentLocalizes In The Lamellipodium In A Fam89b/Lrap25-Dependent Manner |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Mrck Alpha antibodyAnti-Cdc42-Binding Protein Kinase Alpha antibodyAnti-Dmpk-Like Alpha antibodyAnti-Myotonic Dystrophy Kinase-Related Cdc42-Binding Kinase Alpha antibodyAnti-Mrck Alpha antibodyAnti-Myotonic Dystrophy Protein Kinase-Like Alpha antibodyAnti-CDC42BPA antibodyAnti-KIAA0451 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
