Anti-Cdc42-binding kinase alpha antibody (N-Term) (STJ70546)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-Cdc42-binding kinase alpha (N-Term) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.IHC: Paraffin embedded Human Brain (Cerebellum) and Small Intestine (submucosal plexus). Recommended concentration: 3.75µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | CDC42BPA |
| Gene ID | 8476 |
| Uniprot ID | MRCKA_HUMAN |
| Accession Number | NP_055641.3; NP_003598.2 |
| Immunogen Region | N-Term |
| Immunogen Sequence | SGEVRLRQLEQFI |
| Specificity | This antibody is expected to recognise both reported Human isoforms (NP_055641.3 and NP_003598.2). |
Additional Info
| Post Translational Modifications | Proteolytically cleaved by caspases upon apoptosis induction. The cleavage at Asp-478 by CASP3 increases its kinase activity (in vitro). |
| Function | Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake. In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation. Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells. |
| Protein Name | Serine/Threonine-Protein Kinase Mrck AlphaCdc42-Binding Protein Kinase AlphaDmpk-Like AlphaMyotonic Dystrophy Kinase-Related Cdc42-Binding Kinase AlphaMrck AlphaMyotonic Dystrophy Protein Kinase-Like Alpha |
| Database Links | Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013406Reactome: R-HSA-9013409 |
| Cellular Localisation | CytoplasmCell ProjectionLamellipodiumDisplays A Dispersed Punctate Distribution And Concentrates Along The Cell PeripheryEspecially At The Leading Edge And Cell-Cell JunctionThis Concentration Is Ph-Domain DependentLocalizes In The Lamellipodium In A Fam89b/Lrap25-Dependent Manner |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Mrck Alpha antibodyAnti-Cdc42-Binding Protein Kinase Alpha antibodyAnti-Dmpk-Like Alpha antibodyAnti-Myotonic Dystrophy Kinase-Related Cdc42-Binding Kinase Alpha antibodyAnti-Mrck Alpha antibodyAnti-Myotonic Dystrophy Protein Kinase-Like Alpha antibodyAnti-CDC42BPA antibodyAnti-KIAA0451 antibody |
Information sourced from Uniprot.org