• Western blot analysis of extracts of various cell lines, using CCT5 antibody (STJ28632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CCT5 antibody (1-270) (STJ28632)

SKU:
STJ28632

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCT5 (1-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCT5
Gene ID: 22948
Uniprot ID: TCPE_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CCT5 (NP_036205.1).
Immunogen Sequence: MASMGTLAFDEYGRPFLIIK DQDRKSRLMGLEALKSHIMA AKAVANTMRTSLGPNGLDKM MVDKDGDVTVTNDGATILSM MDVDHQIAKLMVELSKSQDD EIGDGTTGVVVLAGALLEEA EQLLDRGIHPIRIADGYEQA ARVAIEHLDKISDSVLVDIK DTEPLIQTAKTTLGSKVVNS CHRQMAEIAVNAVLTVADME RRDVDFELIKVEGKVGGRLE DTKLIKGVIVDKDFSHPQM
Post Translational Modifications Ubiquitinated by the DCX(DCAF12) complex specifically recognizes the diglutamate (Glu-Glu) at the C-terminus, leading to its degradation.
Function Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).
Protein Name T-Complex Protein 1 Subunit Epsilon
Tcp-1-Epsilon
Cct-Epsilon
Database Links Reactome: R-HSA-389957
Reactome: R-HSA-389960
Reactome: R-HSA-390450
Reactome: R-HSA-390471
Reactome: R-HSA-5620922
Reactome: R-HSA-6814122
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Alternative Antibody Names Anti-T-Complex Protein 1 Subunit Epsilon antibody
Anti-Tcp-1-Epsilon antibody
Anti-Cct-Epsilon antibody
Anti-CCT5 antibody
Anti-CCTE antibody
Anti-KIAA0098 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance