• Western blot analysis of extracts of various cell lines, using TCP1 beta/TCP1 beta/CCT2 antibody (STJ28629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Immunofluorescence analysis of HeLa cells using TCP1 beta/TCP1 beta/CCT2 antibody (STJ28629). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug TCP1 beta/TCP1 beta/CCT2 antibody (STJ28629). Western blot was performed from the immunoprecipitate using TCP1 beta/TCP1 beta/CCT2 antibody (STJ28629) at a dilition of 1:1000.

Anti-CCT2 antibody (206-535) (STJ28629)

SKU:
STJ28629

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCT2 (206-535) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCT2
Gene ID: 10576
Uniprot ID: TCPB_HUMAN
Immunogen Region: 206-535
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 206-535 of human TCP1 beta/TCP1 beta/CCT2 (NP_006422.1).
Immunogen Sequence: GGSLADSYLDEGFLLDKKIG VNQPKRIENAKILIANTGMD TDKIKIFGSRVRVDSTAKVA EIEHAEKEKMKEKVERILKH GINCFINRQLIYNYPEQLFG AAGVMAIEHADFAGVERLAL VTGGEIASTFDHPELVKLGS CKLIEEVMIGEDKLIHFSGV ALGEACTIVLRGATQQILDE AERSLHDALCVLAQTVKDSR TVYGGGCSEMLMAHAVTQLA NRTPGKEAVAMESYAKALR
Function Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).
Protein Name T-Complex Protein 1 Subunit Beta
Tcp-1-Beta
Cct-Beta
Database Links Reactome: R-HSA-389957
Reactome: R-HSA-389960
Reactome: R-HSA-390450
Reactome: R-HSA-390471
Reactome: R-HSA-5620922
Reactome: R-HSA-6798695
Reactome: R-HSA-6814122
Reactome: R-HSA-9013418
Reactome: R-HSA-9013422
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-T-Complex Protein 1 Subunit Beta antibody
Anti-Tcp-1-Beta antibody
Anti-Cct-Beta antibody
Anti-CCT2 antibody
Anti-99D8.1 antibody
Anti-CCTB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance