• Immunohistochemistry analysis of CAND2 in paraffin-embedded Human colon carcinoma using CAND2 Rabbit polyclonal antibody (STJ118927) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CAND2 in paraffin-embedded Rat testis using CAND2 Rabbit polyclonal antibody (STJ118927) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CAND2 in paraffin-embedded Mouse spleen using CAND2 Rabbit polyclonal antibody (STJ118927) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CAND2 Rabbit polyclonal antibody (STJ118927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-CAND2 antibody (850-1000) (STJ118927)

SKU:
STJ118927

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CAND2 (850-1000) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAND2
Gene ID: 23066
Uniprot ID: CAND2_HUMAN
Immunogen Region: 850-1000
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 850-1000 of human CAND2 (NP_001155971.1).
Immunogen Sequence: VGQVAGPGHQRELKAVLLEA LGSPSEDVRAAASYALGRVG AGSLPDFLPFLLEQIEAEPR RQYLLLHSLREALGAAQPDS LKPYAEDIWALLFQRCEGAE EGTRGVVAECIGKLVLVNPS FLLPRLRKQLAAGRPHTRST VITAVKFLISD
Tissue Specificity Expressed in epididymis (at protein level).
Post Translational Modifications Ubiquitinated and targeted for proteasomal degradation.
Function Probable assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes.
Protein Name Cullin-Associated Nedd8-Dissociated Protein 2
Cullin-Associated And Neddylation-Dissociated Protein 2
Epididymis Tissue Protein Li 169
Tbp-Interacting Protein Of 120 Kda B
Tbp-Interacting Protein 120b
P120 Cand2
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cullin-Associated Nedd8-Dissociated Protein 2 antibody
Anti-Cullin-Associated And Neddylation-Dissociated Protein 2 antibody
Anti-Epididymis Tissue Protein Li 169 antibody
Anti-Tbp-Interacting Protein Of 120 Kda B antibody
Anti-Tbp-Interacting Protein 120b antibody
Anti-P120 Cand2 antibody
Anti-CAND2 antibody
Anti-KIAA0667 antibody
Anti-TIP120B antibody

Information sourced from Uniprot.org

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