• Western blot analysis of extracts of NIH/3T3 cells, using C14orf166 antibody (STJ26297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.
  • Western blot analysis of extracts of various cell lines, using C14orf166 antibody (STJ26297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-C14orf166 antibody (1-244) (STJ26297)

SKU:
STJ26297

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-C14orf166 (1-244) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RTRAF
Gene ID: 51637
Uniprot ID: RTRAF_HUMAN
Immunogen Region: 1-244
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human C14orf166 (NP_057123.1).
Immunogen Sequence: MFRRKLTALDYHNPAGFNCK DETEFRNFIVWLEDQKIRHY KIEDRGNLRNIHSSDWPKFF EKYLRDVNCPFKIQDRQEAI DWLLGLAVRLEYGDNAEKYK DLVPDNSKTADNATKNAEPL INLDVNNPDFKAGVMALANL LQIQRHDDYLVMLKAIRILV QERLTQDAVAKANQTKEGLP VALDKHILGFDTGDAVLNEA AQILRLLHIEELRELQTKIN EAIVAVQAIIADPKTDHRL
Tissue Specificity Widely expressed. Expressed at high level in heart and skeletal muscle. Expressed at intermediate level in liver, pancreas, fetal brain and fetal lung. Weakly expressed in adult brain, adult lung, placenta, fetal liver and fetal kidney. Overexpressed in many brain tumors.
Function RNA-binding protein involved in modulation of mRNA transcription by Polymerase II. Component of the tRNA-splicing ligase complex and is required for tRNA ligation. May be required for RNA transport. (Microbial infection) In case of infection by influenza virus A (IVA), is involved in viral replication.
Protein Name Rna Transcription - Translation And Transport Factor Protein
Cle7 Homolog
Cle
Hcle
Database Links Reactome: R-HSA-6784531
Cellular Localisation Nucleus
Cytoplasm
Cytosol
Perinuclear Region
Cytoskeleton
Microtubule Organizing Center
Centrosome
May Localize At The Centrosome During Mitosis
Shuttles Between The Cytosol And The Nucleus: Enters Into The Nucleus In Case Of Active Transcription While It Accumulates In Cytosol When Transcription Level Is Low
(Microbial Infection) Following Influenza A Virus (Iav) Infection
Included In Influenza A Virions Via Its Association With Packaged Viral Ribonucleoproteins (Vrnp) In The Nucleus And Cytoplasm
Alternative Antibody Names Anti-Rna Transcription - Translation And Transport Factor Protein antibody
Anti-Cle7 Homolog antibody
Anti-Cle antibody
Anti-Hcle antibody
Anti-RTRAF antibody
Anti-C14orf166 antibody
Anti-CGI-99 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance