| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-C14orf166 (1-244) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RTRAF |
| Gene ID: |
51637 |
| Uniprot ID: |
RTRAF_HUMAN |
| Immunogen Region: |
1-244 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human C14orf166 (NP_057123.1). |
| Immunogen Sequence: |
MFRRKLTALDYHNPAGFNCK DETEFRNFIVWLEDQKIRHY KIEDRGNLRNIHSSDWPKFF EKYLRDVNCPFKIQDRQEAI DWLLGLAVRLEYGDNAEKYK DLVPDNSKTADNATKNAEPL INLDVNNPDFKAGVMALANL LQIQRHDDYLVMLKAIRILV QERLTQDAVAKANQTKEGLP VALDKHILGFDTGDAVLNEA AQILRLLHIEELRELQTKIN EAIVAVQAIIADPKTDHRL |
| Tissue Specificity | Widely expressed. Expressed at high level in heart and skeletal muscle. Expressed at intermediate level in liver, pancreas, fetal brain and fetal lung. Weakly expressed in adult brain, adult lung, placenta, fetal liver and fetal kidney. Overexpressed in many brain tumors. |
| Function | RNA-binding protein involved in modulation of mRNA transcription by Polymerase II. Component of the tRNA-splicing ligase complex and is required for tRNA ligation. May be required for RNA transport. (Microbial infection) In case of infection by influenza virus A (IVA), is involved in viral replication. |
| Protein Name | Rna Transcription - Translation And Transport Factor ProteinCle7 HomologCleHcle |
| Database Links | Reactome: R-HSA-6784531 |
| Cellular Localisation | NucleusCytoplasmCytosolPerinuclear RegionCytoskeletonMicrotubule Organizing CenterCentrosomeMay Localize At The Centrosome During MitosisShuttles Between The Cytosol And The Nucleus: Enters Into The Nucleus In Case Of Active Transcription While It Accumulates In Cytosol When Transcription Level Is Low(Microbial Infection) Following Influenza A Virus (Iav) InfectionIncluded In Influenza A Virions Via Its Association With Packaged Viral Ribonucleoproteins (Vrnp) In The Nucleus And Cytoplasm |
| Alternative Antibody Names | Anti-Rna Transcription - Translation And Transport Factor Protein antibodyAnti-Cle7 Homolog antibodyAnti-Cle antibodyAnti-Hcle antibodyAnti-RTRAF antibodyAnti-C14orf166 antibodyAnti-CGI-99 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and LRRC59 knockout (KO) 293T cells, using [KO Validated] LRRC59 Rabbit polyclonal antibody (STJ11100841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89775/358247/STJ11100841_1__11256.1713122582.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and LRRC59 knockout (KO) 293T cells, using [KO Validated] LRRC59 Rabbit polyclonal antibody (STJ11100841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
