• Western blot analysis of various lysates using BTBD7 Rabbit polyclonal antibody (STJ11100164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-BTBD7 antibody (5-75) (STJ11100164)

SKU:
STJ11100164

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BTBD7 (5-75) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BTBD7
Gene ID: 55727
Uniprot ID: BTBD7_HUMAN
Immunogen Region: 5-75
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 5-75 of human BTBD7 (NP_060637.1).
Immunogen Sequence: ASNYPHSCSPRVGGNSQAQQ TFIGTSSYSQQGYGCESKLY SLDHGHEKPQDKKKRTSGLA TLKKKFIKRRK
Function Acts as a mediator of epithelial dynamics and organ branching by promoting cleft progression. Induced following accumulation of fibronectin in forming clefts, leading to local expression of the cell-scattering SNAIL2 and suppression of E-cadherin levels, thereby altering cell morphology and reducing cell-cell adhesion. This stimulates cell separation at the base of forming clefts by local, dynamic intercellular gap formation and promotes cleft progression.
Protein Name Btb/Poz Domain-Containing Protein 7
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Btb/Poz Domain-Containing Protein 7 antibody
Anti-BTBD7 antibody
Anti-KIAA1525 antibody

Information sourced from Uniprot.org

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