Anti-BID antibody (1-195) [S5057RM] (STJ11105057)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Bh3-Interacting Domain Death Agonist (1-195) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5057RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BID |
| Gene ID | 637 |
| Uniprot ID | BID_HUMAN |
| Immunogen Region | 1-195 |
| Immunogen Sequence | MDCEVNNGSSLRDECITNLL VFGFLQSCSDNSFRRELDAL GHELPVLAPQWEGYDELQTD GNRSSHSRLGRIEADSESQE DIIRNIARHLAQVGDSMDRS IPPGLVNGLALQLRNTSRSE EDRNRDLATALEQLLQAYPR DMEKEKTMLVLALLLAKKVA SHTPSLLRDVFHTTVNFINQ NLRTYVRSLARNGMD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human Bid (NP_001187.1). |
Additional Info
| Tissue Specificity | Isoform 2: Expressed in spleen, pancreas and placenta (at protein level). Isoform 3: Expressed in lung, pancreas and spleen (at protein level). Isoform 4: Expressed in lung and pancreas (at protein level). |
| Post Translational Modifications | BH3-interacting domain death agonist: TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products. Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis. BH3-interacting domain death agonist p15: Ubiquitinated by ITCH.ubiquitination results in proteasome-dependent degradation. |
| Function | Induces caspases and apoptosis. Counters the protective effect of BCL2. BH3-interacting domain death agonist p15: Induces caspase activation and apoptosis. Allows the release of cytochrome c. Isoform 1: Induces ICE-like proteases and apoptosis. Isoform 2: Induces ICE-like proteases and apoptosis. Isoform 3: Does not induce apoptosis. Isoform 4: Induces ICE-like proteases and apoptosis. |
| Protein Name | Bh3-Interacting Domain Death AgonistP22 BidBid Cleaved Into - Bh3-Interacting Domain Death Agonist P15P15 Bid - Bh3-Interacting Domain Death Agonist P13P13 Bid - Bh3-Interacting Domain Death Agonist P11P11 Bid |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-111452Reactome: R-HSA-111453Reactome: R-HSA-114294Reactome: R-HSA-6803204Reactome: R-HSA-75108 |
| Cellular Localisation | CytoplasmMitochondrion MembraneMitochondrion Outer MembraneWhen UncleavedIt Is Predominantly CytoplasmicBh3-Interacting Domain Death Agonist P15: Mitochondrion MembraneTranslocates To Mitochondria As An Integral Membrane ProteinBh3-Interacting Domain Death Agonist P13: Mitochondrion MembraneAssociated With The Mitochondrial MembraneIsoform 1: CytoplasmIsoform 3: CytoplasmIsoform 2: Mitochondrion MembraneA Significant Proportion Of Isoform 2 Localizes To MitochondriaIt May Be Cleaved Constitutively |
| Alternative Antibody Names | Anti-Bh3-Interacting Domain Death Agonist antibodyAnti-P22 Bid antibodyAnti-Bid Cleaved Into - Bh3-Interacting Domain Death Agonist P15 antibodyAnti-P15 Bid - Bh3-Interacting Domain Death Agonist P13 antibodyAnti-P13 Bid - Bh3-Interacting Domain Death Agonist P11 antibodyAnti-P11 Bid antibodyAnti-BID antibody |
Information sourced from Uniprot.org