• Immunohistochemistry analysis of paraffin-embedded mouse pancreas using ATG5 antibody (STJ113131) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat pancreas using ATG5 antibody (STJ113131) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using ATG5 antibody (STJ113131) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of SH-SY5Y, using ATG5 Rabbit polyclonal antibody (STJ113131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ATG5 antibody (28-275) (STJ113131)

SKU:
STJ113131

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATG5 (28-275) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATG5
Gene ID: 9474
Uniprot ID: ATG5_HUMAN
Immunogen Region: 28-275
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-275 of human ATG5 (NP_004840.1).
Immunogen Sequence: TEREAEPYYLLLPRVSYLTL VTDKVKKHFQKVMRQEDISE IWFEYEGTPLKWHYPIGLLF DLLASSSALPWNITVHFKSF PEKDLLHCPSKDAIEAHFMS CMKEADALKHKSQVINEMQK KDHKQLWMGLQNDRFDQFWA INRKLMEYPAEENGFRYIPF RIYQTTTERPFIQKLFRPVA ADGQLHTLGDLLKEVCPSAI DPEDGEKKNQVMIHGIEPML ETPLQWLSEHLSYPDNFLH
Tissue Specificity Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
Post Translational Modifications Conjugated to ATG12.which is essential for autophagy, but is not required for association with isolation membrane. Acetylated by EP300.
Function Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD. (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established.
Protein Name Autophagy Protein 5
Apg5-Like
Apoptosis-Specific Protein
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-5205685
Reactome: R-HSA-8934903
Reactome: R-HSA-936440
Cellular Localisation Cytoplasm
Preautophagosomal Structure Membrane
Peripheral Membrane Protein
Colocalizes With Nonmuscle Actin
The Conjugate Detaches From The Membrane Immediately Before Or After Autophagosome Formation Is Completed
Localizes Also To Discrete Punctae Along The Ciliary Axoneme And To The Base Of The Ciliary Axoneme
Alternative Antibody Names Anti-Autophagy Protein 5 antibody
Anti-Apg5-Like antibody
Anti-Apoptosis-Specific Protein antibody
Anti-ATG5 antibody
Anti-APG5L antibody
Anti-ASP antibody

Information sourced from Uniprot.org

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