| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ATG5 (28-275) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATG5 |
| Gene ID: |
9474 |
| Uniprot ID: |
ATG5_HUMAN |
| Immunogen Region: |
28-275 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 28-275 of human ATG5 (NP_004840.1). |
| Immunogen Sequence: |
TEREAEPYYLLLPRVSYLTL VTDKVKKHFQKVMRQEDISE IWFEYEGTPLKWHYPIGLLF DLLASSSALPWNITVHFKSF PEKDLLHCPSKDAIEAHFMS CMKEADALKHKSQVINEMQK KDHKQLWMGLQNDRFDQFWA INRKLMEYPAEENGFRYIPF RIYQTTTERPFIQKLFRPVA ADGQLHTLGDLLKEVCPSAI DPEDGEKKNQVMIHGIEPML ETPLQWLSEHLSYPDNFLH |
| Tissue Specificity | Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. |
| Post Translational Modifications | Conjugated to ATG12.which is essential for autophagy, but is not required for association with isolation membrane. Acetylated by EP300. |
| Function | Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD. (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established. |
| Protein Name | Autophagy Protein 5Apg5-LikeApoptosis-Specific Protein |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-5205685Reactome: R-HSA-8934903Reactome: R-HSA-936440 |
| Cellular Localisation | CytoplasmPreautophagosomal Structure MembranePeripheral Membrane ProteinColocalizes With Nonmuscle ActinThe Conjugate Detaches From The Membrane Immediately Before Or After Autophagosome Formation Is CompletedLocalizes Also To Discrete Punctae Along The Ciliary Axoneme And To The Base Of The Ciliary Axoneme |
| Alternative Antibody Names | Anti-Autophagy Protein 5 antibodyAnti-Apg5-Like antibodyAnti-Apoptosis-Specific Protein antibodyAnti-ATG5 antibodyAnti-APG5L antibodyAnti-ASP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
