• Immunofluorescence analysis of L929 cells using ASPM Rabbit polyclonal antibody (STJ11100117) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ASPM Rabbit polyclonal antibody (STJ11100117) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ASPM Rabbit polyclonal antibody (STJ11100117) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ASPM Rabbit polyclonal antibody (STJ11100117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ASPM antibody (450-550) (STJ11100117)

SKU:
STJ11100117

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ASPM (450-550) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ASPM
Gene ID: 259266
Uniprot ID: ASPM_HUMAN
Immunogen Region: 450-550
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 450-550 of human ASPM (NP_060606.3).
Immunogen Sequence: IFEELVEMKSNYYSFIKQNN PKFSAVQDISSHSHNKQPKR RPILSATVTKRKATCTRENQ TEINKPKAKRCLNSAVGEHE KVINNQKEKEDFHSYLPIID P
Function Involved in mitotic spindle regulation and coordination of mitotic processes. The function in regulating microtubule dynamics at spindle poles including spindle orientation, astral microtubule density and poleward microtubule flux seems to depend on the association with the katanin complex formed by KATNA1 and KATNB1. Enhances the microtubule lattice severing activity of KATNA1 by recruiting the katanin complex to microtubules. Can block microtubule minus-end growth and reversely this function can be enhanced by the katanin complex. May have a preferential role in regulating neurogenesis.
Protein Name Abnormal Spindle-Like Microcephaly-Associated Protein
Abnormal Spindle Protein Homolog
Asp Homolog
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle
Nucleus
The Nuclear-Cytoplasmic Distribution Could Be Regulated By The Availability Of Calmodulin
Localizes To Spindle Poles During Mitosis
Associates With Microtubule Minus Ends
Alternative Antibody Names Anti-Abnormal Spindle-Like Microcephaly-Associated Protein antibody
Anti-Abnormal Spindle Protein Homolog antibody
Anti-Asp Homolog antibody
Anti-ASPM antibody
Anti-MCPH5 antibody

Information sourced from Uniprot.org

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