• Immunohistochemistry analysis of ANKLE2 in paraffin-embedded Human lung cancer using ANKLE2 Rabbit polyclonal antibody (STJ119707) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ANKLE2 in paraffin-embedded Rat testis using ANKLE2 Rabbit polyclonal antibody (STJ119707) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ANKLE2 in paraffin-embedded Mouse kidney using ANKLE2 Rabbit polyclonal antibody (STJ119707) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ANKLE2 Rabbit polyclonal antibody (STJ119707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ANKLE2 antibody (350-450) (STJ119707)

SKU:
STJ119707

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANKLE2 (350-450) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANKLE2
Gene ID: 23141
Uniprot ID: ANKL2_HUMAN
Immunogen Region: 350-450
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 350-450 of human ANKLE2 (NP_055929.1).
Immunogen Sequence: CRYNVMHVAAKENQASICQL TLDVLENPDFMRLMYPDDDE AMLQKRIRYVVDLYLNTPDK MGYDTPLHFACKFGNADVVN VLSSHHLIVKNSRNKYDKTP E
Function Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. May regulate nuclear localization of VRK1 in non-dividing cells. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex. Involved in brain development.
Protein Name Ankyrin Repeat And Lem Domain-Containing Protein 2
Lem Domain-Containing Protein 4
Database Links Reactome: R-HSA-2995383
Reactome: R-HSA-9013404
Reactome: R-HSA-9013408
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iii Membrane Protein
Alternative Antibody Names Anti-Ankyrin Repeat And Lem Domain-Containing Protein 2 antibody
Anti-Lem Domain-Containing Protein 4 antibody
Anti-ANKLE2 antibody
Anti-KIAA0692 antibody
Anti-LEM4 antibody

Information sourced from Uniprot.org

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