• Immunohistochemistry analysis of AMOTL2 in paraffin-embedded human stomach using AMOTL2 Rabbit polyclonal antibody (STJ119134) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of AMOTL2 in paraffin-embedded human liver cancer using AMOTL2 Rabbit polyclonal antibody (STJ119134) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from BeWo cells using AMOTL2 Rabbit polyclonal antibody (STJ119134) at 1:600 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:60s.

Anti-AMOTL2 antibody (638-837) (STJ119134)

SKU:
STJ119134

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AMOTL2 (638-837) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AMOTL2
Gene ID: 51421
Uniprot ID: AMOL2_HUMAN
Immunogen Region: 638-837
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 638-837 of human AMOTL2 (NP_001265612.1).
Immunogen Sequence: RQFAMDAAATAAAQRDTTLI RHSPQPSPSSSFNEGLLTGG HRHQEMESRLKVLHAQILEK DAVIKVLQQRSRRDPGKAIQ GSLRPAKSVPSVFAAAAAGT QGWQGLSSSERQTADAPARL TTDRAPTEEPVVTAPPAAHA KHGSRDGSTQTEGPPDSTST CLPPEPDSLLGCSSSQRAAS LDSVATSRVQDLSDMVEILI
Post Translational Modifications Phosphorylation at Tyr-107 is necessary for efficient binding to SRC and synergistically functioning with SRC to activate the downstream MAPK pathway.
Function Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. May play a role in the polarity, proliferation and migration of endothelial cells. Selectively promotes FGF-induced MAPK activation through SRC.
Protein Name Angiomotin-Like Protein 2
Leman Coiled-Coil Protein
Lccp
Database Links Reactome: R-HSA-2028269
Cellular Localisation Recycling Endosome
Alternative Antibody Names Anti-Angiomotin-Like Protein 2 antibody
Anti-Leman Coiled-Coil Protein antibody
Anti-Lccp antibody
Anti-AMOTL2 antibody
Anti-KIAA0989 antibody

Information sourced from Uniprot.org

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